Effect of serine protease inhibitor, N-alpha-tosyl-L-lysyl-chloromethyl ketone (TLCK), on cell-mediated and cell-free HIV-1 spread.

Abstract

The effect of a serine protease inhibitor, N-alpha-tosyl-L-lysyl-chloromethyl ketone (TLCK), on cell adhesion and resulting cell-to-cell HIV-1 transmission was examined. Cell-mediated viral spread was blocked by 10(-4) M TLCK--the nontoxic dose at which the maximum inhibition of cell adhesion was achieved. TLCK (10(-4)-10(-8) M range) exhibited a similar dose-dependent effect when tested against cell-free virus infection. These findings suggest that TLCK acts by preventing both cell-cell adhesion and viral binding to the target cell. The effect of TLCK could be attributed to an irreversible modification of surface and/or intracellular proteases required for functional activation of HIV-1 envelope glycoproteins.

• Authors: A S Bourinbaiar, R Nagorny
• Journal: Cellular immunology (Cell Immunol) Vol. 155 Issue 1 Pg. 230-6 (Apr 15 1994) ISSN: 0008-8749 [Print] Netherlands
• PMID: 8168146 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Tosyllysine Chloromethyl Ketone

Topics

• Cell Adhesion (drug effects)
• Dose-Response Relationship, Drug
• HIV-1 (growth & development)
• Humans
• T-Lymphocytes (microbiology)
• Tosyllysine Chloromethyl Ketone (pharmacology)
• Virus Replication (drug effects)