[Studies on ras P21 expression and mutation at 12th codon of C-Ha-ras oncogene in squamous cell carcinoma of the larynx].

Abstract

Monoclonal antibody directed against the ras oncogene product P21 was used to evaluate P21 expression in 42 cases of tumors of the larynx. The results showed that there was a higher level of ras P21 expression in squamous cell carcinoma of the larynx (LSCC) than in papilloma of the larynx (LP) and the normal tissues adjacent to LSCC, P < 0.05. We had also detected G-->T mutation at codon 12 of Ha-ras oncogenes in LSCC using polymerase chain reaction (PCR). PCR products were hybridized with mutation-specific oligonucleotide probes. Thirty per cent (6/20) LSCC cases harbored this mutation. But G-->T mutation was absent in 3 LP and 5 normal tissues adjacent to LSCC. A relationship may exist between the presence of G-->T mutation and Jakobsson's malignant grading result. All of 6 LSCC cases containing G-->T mutation were in high malignant grading group, P < 0.05. This study implicates that overexpression of ras P21 and presence of Ha-ras G-->T mutation may be one of the molecular mechanisms of maintenance of malignancy and progression of LSCC.

• Authors: Q Yang
• Journal: Zhonghua er bi yan hou ke za zhi (Zhonghua Er Bi Yan Hou Ke Za Zhi) Vol. 28 Issue 4 Pg. 209-12, 251 ( 1993) ISSN: 0412-3948 [Print] China
• PMID: 8167038 (Publication Type: English Abstract, Journal Article)

Chemical References

• Codon
• DNA, Neoplasm
• Oncogene Protein p21(ras)

Topics

• Base Sequence
• Carcinoma, Squamous Cell (genetics, metabolism)
• Codon (genetics)
• DNA, Neoplasm (analysis)
• Gene Expression
• Genes, ras
• Humans
• Laryngeal Neoplasms (genetics, metabolism)
• Molecular Sequence Data
• Oncogene Protein p21(ras) (metabolism)
• Point Mutation
• Polymerase Chain Reaction