Effects of spermidine on function and ultrastructure of alveolar macrophages.

Abstract

It is known that spermidine and other naturally occurring polyamines accumulate in rat lung slices by an energy-dependent uptake process and that alveolar macrophages (AM) have a greater rate of uptake than has the total lung cell population. In the present study rat AMs were incubated with spermidine, which resulted in a marked and significant (P < 0.002) decrease in phagocytosis of heat-killed yeast cells at concentrations 0.2 and 0.5 mM and a tendency to decrease at 0.05 mM. The number of microtubules surrounding the centrioles was measured using electron microscopy and appeared to be decreased at concentrations 0.2 and 0.5 mM. There was no affect on phagolysosomal pH. The results suggest that spermidine might affect the defense against inhaled pollutants and microbes, especially when spermidine levels are increased, as they are under conditions with high mitotic activity, e.g., in tumors.

• Authors: A G Bulychev, A Johansson, M Lundborg, P Camner, B A Afzelius
• Journal: Experimental and molecular pathology (Exp Mol Pathol) Vol. 60 Issue 1 Pg. 52-9 (Feb 1994) ISSN: 0014-4800 [Print] Netherlands
• PMID: 8162970 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Spermidine

Topics

• Animals
• Dose-Response Relationship, Drug
• Hydrogen-Ion Concentration
• Lysosomes (drug effects)
• Macrophages, Alveolar (drug effects, ultrastructure)
• Male
• Microscopy, Electron
• Phagocytosis (drug effects)
• Rats
• Rats, Sprague-Dawley
• Spermidine (pharmacology)