Immunoglobulin prophylaxis against human T cell lymphotropic virus type II in rabbits.

Abstract

Hyperimmune globulins, I-IgG and II-IgG, prepared from healthy persons seropositive for human T cell lymphotropic virus (HTLV) types I and II, respectively, were tested for their prophylactic effect against HTLV-II infection in rabbits. Three groups (A, B, and C) of 3 female rabbits each were used. Group A rabbits were inoculated intravenously with HTLV-II-carrying rabbit lymphoid cell line RII. Group B and C rabbits were immunized, respectively, with II-IgG and I-IgG and challenged with RII cells 24 h later. All group A and C rabbits seroconverted for HTLV-II after 2 weeks, but all group B rabbits were protected from HTLV-II infection. Gene amplification by polymerase chain reaction revealed the presence of HTLV-II provirus sequences in all group A and C rabbits but in none of the group B rabbits. These findings indicate that passive immunization with II-IgG is effective in preventing HTLV-II infection and that there is no cross-neutralization between HTLV-I and -II.

• Authors: N Morishita, K Ishii, Y Tanaka, T Sawada, H Hoshino, S K Foung, I Miyoshi
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 169 Issue 3 Pg. 620-3 (Mar 1994) ISSN: 0022-1899 [Print] United States
• PMID: 8158037 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• HTLV-II Antibodies
• Immunoglobulin G

Topics

• Animals
• Blotting, Western
• Cell Line
• Female
• HTLV-II Antibodies (immunology, therapeutic use)
• HTLV-II Infections (immunology, prevention & control)
• Humans
• Immunoglobulin G (therapeutic use)
• Neutralization Tests
• Polymerase Chain Reaction
• Rabbits