Abstract |
A method is described for the determination of the antitumour drug cyclophosphamide and six stable metabolites in plasma of cancer patients, namely dechloroethyl- cyclophosphamide, 4-keto-cyclophosphamide, carboxy- phosphamide, alcophosphamide, nor-nitrogen mustard and the N-chloroethyl-1,3-oxazolidine-2-one, as methyl and/or trifluoroacetyl derivatives by single ion monitoring gas chromatography/mass spectrometry, mostly in the electron capture chemical ionization mode. The isolation of most metabolites was performed by solid-phase C-18 extraction in weakly acidic medium. The phosphoramide mustard isolated under these conditions decomposes readily to the nor-nitrogen mustard during derivatization. The original nor-nitrogen mustard and the chloroethyl-1,3-oxazolidine-2-one were isolated by liquid extraction with ethyl acetate in alkaline medium. Recoveries of 75-99% were measured using spiked blank plasma samples. Quantitation of metabolites in patient plasma samples was performed using two sets of calibration curves for the concentration ranges of 1-100 ng and 0.1-10 micrograms of metabolite per millilitre of original plasma.
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Authors | G Momerency, K Van Cauwenberghe, P H Slee, A T Van Oosterom, E A De Bruijn |
Journal | Biological mass spectrometry
(Biol Mass Spectrom)
Vol. 23
Issue 3
Pg. 149-58
(Mar 1994)
ISSN: 1052-9306 [Print] England |
PMID | 8148406
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Nitrogen Mustard Compounds
- Oxazoles
- Oxazolidinones
- Phosphoramide Mustards
- N-chloroethyl-1,3-oxazolidin-2-one
- dechloroethylcyclophosphamide
- alcophosphamide
- Cyclophosphamide
- carboxyphosphamide
- nornitrogen mustard
- 4-ketocyclophosphamide
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Topics |
- Antineoplastic Agents
(therapeutic use)
- Cyclophosphamide
(analogs & derivatives, blood, therapeutic use)
- Gas Chromatography-Mass Spectrometry
- Humans
- Neoplasms
(drug therapy)
- Nitrogen Mustard Compounds
(blood)
- Oxazoles
(blood)
- Oxazolidinones
- Phosphoramide Mustards
(blood)
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