Cholesterol in remnant-like lipoproteins in human serum using monoclonal anti apo B-100 and anti apo A-I immunoaffinity mixed gels.

Abstract

We have developed a simple, rapid assay method for apo E-rich lipoproteins (d < 1.006 g/ml), using an immunoaffinity gel mixture of anti apo B-100 and apo A-I antibodies coupled to Sepharose 4B. The immunoaffinity mixed gels adsorb normal lipoproteins containing apo A-I quantitatively as well as most lipoproteins containing apo B-100. Unbound lipoproteins are quantified by assay of cholesterol. Characterization of the unbound lipoproteins of d < 1.006 g/ml (J Lipid Res 1992; 33: 369-380) has shown that they represent chylomicron and VLDL remnant-like particles (RLP). RLP-Cholesterol(C) levels in plasma have been determined in 363 male and female normolipidemic subjects (mean +/- S.D.: 72 +/- 16 mg/l) and have been found to be higher in patients with coronary heart disease and familial dysbetalipoproteinemia. Triglyceride-rich lipoproteins may well contain both atherogenic and non-atherogenic particles that can be separated by this simple immunoadsorption assay.

• Authors: K Nakajima, T Saito, A Tamura, M Suzuki, T Nakano, M Adachi, A Tanaka, N Tada, H Nakamura, E Campos
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 223 Issue 1-2 Pg. 53-71 (Dec 31 1993) ISSN: 0009-8981 [Print] Netherlands
• PMID: 8143370 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Apolipoprotein A-I
• Apolipoprotein B-100
• Apolipoproteins B
• Chylomicrons
• Gels
• Lipoproteins
• Lipoproteins, VLDL
• Cholesterol

Topics

• Adolescent
• Adult
• Antibodies, Monoclonal
• Apolipoprotein A-I (immunology)
• Apolipoprotein B-100
• Apolipoproteins B (immunology)
• Child
• Child, Preschool
• Cholesterol (blood)
• Chromatography, Affinity
• Chylomicrons (blood)
• Coronary Disease (blood)
• Female
• Gels
• Humans
• Immunoblotting
• Infant
• Lipoproteins (blood, chemistry)
• Lipoproteins, VLDL (blood)
• Male
• Middle Aged