Purification, characterization and biological activities of phospholipase A from Russell's viper (Vipera russelli) venom.

Abstract

1. The major phospholipase A has been purified to electrophoretic homogeneity from the venom of Vipera russelli (Russell's viper). 2. The molecular weight of the purified enzyme was estimated to be 31,000 by Sephadex G-75 gel filtration chromatography and 29,000 by SDS-polyacrylamide gel electrophoresis. The enzyme exhibited an apparent Km value of 2.3 x 10(-2) M. 3. The phospholipase A showed edema forming, indirect hemolytic and myonecrotic activities but not hemorrhagic activity.

• Authors: Aye-Kyaw, San-Aye, Tin-Win, Hla-Pe, Mya-Maung
• Journal: The International journal of biochemistry (Int J Biochem) Vol. 26 Issue 1 Pg. 79-83 (Jan 1994) ISSN: 0020-711X [Print] England
• PMID: 8138052 (Publication Type: Journal Article)

Chemical References

• Viper Venoms
• Phospholipases A

Topics

• Animals
• Edema (chemically induced)
• Hemolysis (drug effects)
• Hemorrhage
• Mice
• Molecular Weight
• Necrosis
• Phospholipases A (chemistry, isolation & purification, toxicity)
• Viper Venoms (enzymology)