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Protection of gerbils from amebic liver abscess by immunization with a recombinant Entamoeba histolytica antigen.

Abstract
Amebiasis, infection by the intestinal protozoan parasite Entamoeba histolytica, is a leading parasitic cause of death. As a step in the development of a recombinant antigen vaccine to prevent E. histolytica infection, we looked at the ability of a recombinant version of the serine-rich E. histolytica protein (SREHP) to elicit a protective immune response against invasive amebic disease. Gerbils, a standard model for amebic liver abscess, were immunized with either a recombinant SREHP/maltose-binding protein (MBP) fusion, recombinant MBP alone, or phosphate-buffered saline (PBS), all combined with complete Freund's adjuvant. In the first trial (group 1), gerbils received a primary and two booster immunizations intraperitoneally; in the second trial (group 2), gerbils were immunized by a single intradermal injection. SREHP/MBP-immunized gerbils in both groups produced antibody to native SHEHP and developed delayed-type hypersensitivity responses to recombinant SREHP. All gerbils were challenged by an intrahepatic injection with 5 x 10(4) virulent E. histolytica HM1-IMSS trophozoites. Complete protection from amebic liver abscess was seen in 64% of the SHEHP/MBP-immunized gerbils in group 1 and in 100% of the SREHP/MBP-immunized gerbils in group 2. There was no protection observed in MBP- or PBS-immunized gerbils in either group. Our results indicate that the SREHP molecule has potential as a vaccine to prevent amebic infection and demonstrate that successful vaccination of animals with recombinant E. histolytica antigen vaccines is possible.
AuthorsT Zhang, P R Cieslak, S L Stanley Jr
JournalInfection and immunity (Infect Immun) Vol. 62 Issue 4 Pg. 1166-70 (Apr 1994) ISSN: 0019-9567 [Print] United States
PMID8132322 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antigens, Protozoan
  • Membrane Proteins
  • Protozoan Proteins
  • Protozoan Vaccines
  • Vaccines, Synthetic
  • serine-rich protein, Entamoeba histolytica
Topics
  • Animals
  • Antigens, Protozoan (immunology)
  • Entamoeba histolytica (immunology)
  • Female
  • Gerbillinae
  • Hypersensitivity, Delayed
  • Immunization
  • Liver Abscess, Amebic (prevention & control)
  • Membrane Proteins (immunology)
  • Protozoan Proteins (immunology)
  • Protozoan Vaccines (immunology)
  • Vaccines, Synthetic (immunology)

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