Intercellular adhesion induced by anti-alpha 3 integrin (VLA-3) antibodies.

Abstract

We generated four monoclonal antibodies (mAbs) specific for human alpha 3 integrin (VLA-3 alpha subunit). All of them were found to induce homotypic cell aggregation of HT1080 fibrosarcoma and SN12C renal carcinoma cells, both of which express high levels of alpha 3 integrin. The antibodies also induced the cell aggregation of K562 erythroleukemic cells transfected with alpha 3 integrin cDNA, but not the parental K562 cells. The aggregation was observed in a temperature-dependent manner and was not inhibited by the addition of EDTA. Immunofluorescence microscopic observation showed that alpha 3 integrin on HT1080 cells was translocated into the contact regions after the mAb treatment. The intercellular adhesion between cells expressing alpha 3 integrin and cells without alpha 3 integrin was also induced by the anti-alpha 3 antibody treatment.

• Authors: K Takeuchi, T Tsuji, S Hakomori, T Irimura
• Journal: Experimental cell research (Exp Cell Res) Vol. 211 Issue 1 Pg. 133-41 (Mar 1994) ISSN: 0014-4827 [Print] United States
• PMID: 8125150 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Receptors, Very Late Antigen

Topics

• Antibodies, Monoclonal (immunology, pharmacology)
• Breast Neoplasms (pathology)
• Cell Adhesion (drug effects, physiology)
• Cell Aggregation (physiology)
• Fibrosarcoma (pathology)
• Humans
• Kidney Neoplasms (pathology)
• Leukemia, Erythroblastic, Acute (pathology)
• Microscopy, Fluorescence
• Receptors, Very Late Antigen (genetics, immunology, physiology)
• Transfection
• Tumor Cells, Cultured