Abstract | OBJECTIVE: DESIGN: Prospective, randomized trial. SETTING: Animal laboratory. SUBJECTS: Domestic pigs aged 7 to 8 wks. INTERVENTIONS: Two groups of pigs were anesthetized with midazolam and sufentanyl, mechanically ventilated, and hemodynamically monitored. In controls (n = 9) and in animals with acute necrotizing pancreatitis (n = 9), a segment of the jejunum was isolated and autoperfused in situ. Through an antimesenteric enterotomy, an area of jejunal mucosa was exposed for mucosal PO2 measurements. Acute necrotizing pancreatitis was induced by the injection of 10 mL of 10% sodium-taurocholate into the main pancreatic duct. Both groups received normal saline solution to keep pulmonary artery occlusion pressure constant. MEASUREMENTS: Mucosal PO2 was assessed with a modified Clark-type multiwire surface electrode. After two baseline measurements, systemic and regional oxygen transport variables and mucosal PO2 were determined at designated intervals (20, 40, 60, 100, 120, 160, 200, 240, 280 mins). MAIN RESULTS: Systemic hemodynamics and oxygen transport were maintained in both groups. In contrast to controls, all animals with pancreatitis showed gross macroscopic and histologic evidence of severe acute necrotizing pancreatitis at autopsy. There were no significant differences between groups in jejunal blood flow, oxygen transport, oxygen consumption, oxygen extraction ratio, or mucosal PO2. CONCLUSIONS:
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Authors | C Schwarz, W Hasibeder, M Haisjackl, R Germann, H Sparr, B Abendstein, B Friesenecker, M Herold |
Journal | Critical care medicine
(Crit Care Med)
Vol. 22
Issue 1
Pg. 135-41
(Jan 1994)
ISSN: 0090-3493 [Print] United States |
PMID | 8124956
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Acute Disease
- Animals
- Hemodynamics
- Jejunum
(blood supply)
- Oxygen
(metabolism)
- Oxygen Consumption
- Pancreatitis
(chemically induced, metabolism, pathology)
- Swine
- Taurocholic Acid
(toxicity)
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