Abstract |
We have discovered a unique group I intron-like insertion (DiSSU) in the nuclear small subunit ribosomal RNA gene of the myxomycete Didymium iridis. By sequence, DiSSU consists of a group I ribozyme at the 5' end, an open reading frame (ORF) in the middle, and a novel element at the 3' end. Intron RNA self-splices in vitro to yield ten major processed RNAs, including a full-length circle. The group I ribozyme can efficiently cleave at an internal processing site, which separates the group I ribozyme from the ORF. Surprisingly, deletion that remove the entire group I ribozyme do not impair cleavage at the 3' splice site, implying that the 3' element itself is a catalytic RNA. Deletions that remove portions of the 3' element prevent utilization of the 5' splice site, suggesting that this element cooperates with the upstream group I ribozyme in splicing. DiSSU appears to be the first example for the cooperative interaction of distinct ribozymes in RNA splicing.
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Authors | S Johansen, V M Vogt |
Journal | Cell
(Cell)
Vol. 76
Issue 4
Pg. 725-34
(Feb 25 1994)
ISSN: 0092-8674 [Print] United States |
PMID | 8124711
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA Primers
- DNA, Fungal
- DNA, Ribosomal
- RNA, Catalytic
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Topics |
- Amino Acid Sequence
- Base Sequence
- DNA Primers
(chemistry)
- DNA, Fungal
(genetics)
- DNA, Ribosomal
- Genes, Fungal
- Hydrogen Bonding
- Introns
- Molecular Sequence Data
- Myxomycetes
(genetics)
- Nucleic Acid Conformation
- Open Reading Frames
- RNA Splicing
- RNA, Catalytic
(genetics)
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