[Wound healing of the corneal endothelium in vivo after intraocular injection of recombinant human bFGF].

Abstract

In the present study, we investigated the potential role of recombinant human basic fibroblast growth factor (rh-bFGF) on the regeneration of corneal endothelium in the rabbit. For in vivo wound-healing experiments, standardized wounds were created by mechanically scraping the endothelial cells within the boundaries of a central epithelial trephine mark. A single dose of 1.5 micrograms rh-bFGF was injected into the anterior chamber of the right eye immediately after wounding, while the left eyes received the ointment vehicle only (n = 27 rabbits). Functional recovery and wound closure rates were assessed by means of ultrasonic pachymetry, cell counts after endothelial vital staining and direct computer-assisted surface analysis of Janus green-stained endothelial lesions. Measurements were carried out 0, 1, 2, 4, and 7 days after injury. For the time points day 2 and day 4, significant stimulation of endothelial regeneration in rh-bFGF treated eyes was observed with all parameters measured. These results demonstrate that rh-bFGF could prove to be useful in clinical conditions, e.g. to conserve endothelial function after intraocular surgery or as a supplement to corneal intermediate preservation media.

• Authors: P Rieck, C Hartmann, Y Pouliquen, Y Courtois
• Journal: Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft (Ophthalmologe) Vol. 90 Issue 6 Pg. 652-5 (Dec 1993) ISSN: 0941-293X [Print] Germany
• Vernacular Title: Wundheilung des Hornhautendothels in vivo nach intraokularer Injektion von rekombinantem humanem bFGF.
• PMID: 8124029 (Publication Type: English Abstract, Journal Article)

Chemical References

• Recombinant Proteins
• Fibroblast Growth Factor 2

Topics

• Animals
• Anterior Chamber
• Cell Count (drug effects)
• Endothelium, Corneal (drug effects, injuries, pathology)
• Female
• Fibroblast Growth Factor 2 (pharmacology)
• Injections
• Male
• Rabbits
• Recombinant Proteins (pharmacology)
• Regeneration (drug effects, physiology)
• Wound Healing (drug effects, physiology)