[Preparation of precipitation antigens for the diagnosis of Maedi-Visna].

Abstract

In the present paper antigen preparation is described to be used for diagnostics of Maedi-Visna disease by immunodiffusion test (IDT). The antigen was prepared by cultivation of the Maedi-Visna virus, OLV strain, in cell culture of the synovial membrane of carpal joint and plexus choroides of lamb. Cell cultures were used for 10-25 subcultures. The antigen was concentrated by polyethylene glycol application. The characteristics of the prepared diagnostic antigen were evaluated by its comparison with a reference, commercial preparation. Sufficient sensitivity and specificity of the prepared antigen was demonstrated by testing in a sample of 100 ovine serums.

• Authors: V Celer Jr
• Journal: Veterinarni medicina (Vet Med (Praha)) Vol. 38 Issue 11 Pg. 641-6 ( 1993) ISSN: 0375-8427 [Print] Czech Republic
• Vernacular Title: Príprava precipitacního antigenu k diagnostice Maedi-Visna.
• PMID: 8116136 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigens, Viral

Topics

• Animals
• Antigens, Viral (biosynthesis)
• Cells, Cultured
• Sheep
• Synovial Membrane
• Virus Cultivation
• Visna-maedi virus (immunology)