Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules.

Abstract

Ly-49 is a recently identified cell surface molecule expressed on a subpopulation of natural killer (NK) cells and certain T lymphomas. It has been suggested, based on gene transfection and antibody blocking studies, that Ly-49 is a negative regulator of NK lytic activity, possibly through an interaction with target cell class I molecules. However, it has not been demonstrated that class I molecules indeed serve as ligands for Ly-49. We have found that T lymphomas expressing Ly-49 bind isolated class I major histocompatibility complex (MHC) molecules but not class II molecules immobilized on plastic. Adhesion to class I molecules was not found with T lymphomas lacking Ly-49 expression. The Ly-49 expressing EL4 lymphoma bound Dd, Dk, and Kb, but not Kd, Kk, or Db, thus demonstrating a restricted pattern of class I adhesion. The observed cell adhesion was class I density dependent, and binding to Dd and Dk was extensively inhibited by the A1 monoclonal antibody directed against Ly-49. These results provide direct evidence for Ly-49 serving as a receptor for a subset of class I MHC molecules.

• Authors: K P Kane
• Journal: The Journal of experimental medicine (J Exp Med) Vol. 179 Issue 3 Pg. 1011-5 (Mar 01 1994) ISSN: 0022-1007 [Print] United States
• PMID: 8113669 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HLA-D Antigens
• Histocompatibility Antigens Class I
• Histocompatibility Antigens Class II
• Membrane Proteins

Topics

• Animals
• Cell Adhesion (immunology)
• Cell Line
• HLA-D Antigens (immunology, metabolism)
• Histocompatibility Antigens Class I (immunology, isolation & purification, metabolism)
• Histocompatibility Antigens Class II (immunology, isolation & purification, metabolism)
• Hybridomas (immunology)
• Killer Cells, Natural (immunology)
• Kinetics
• Lymphoma
• Lymphoma, T-Cell (immunology)
• Membrane Proteins (immunology, metabolism)
• Mice
• Tumor Cells, Cultured