Abstract |
The effects of vanadyl sulfate treatment on susceptibility to oxidative stress were investigated in streptozotocin-diabetic Wistar rats. A 2 x 2 factorial design was employed, with four groups of animals: 1) untreated, non-diabetic; 2) vanadyl-treated, non-diabetic; 3) untreated, diabetic; and 4) vanadyl-treated, diabetic. Vanadyl sulfate was administered as a 1.00 to 1.25 mg/ml solution in drinking water. Cataract development was entirely suppressed in vanadyl-treated compared to untreated, diabetic rats. STZ-induction of diabetes diminished glutathione (GSH) levels in liver homogenates; whereas vanadyl treatment resulted in restored levels of this nonenzymatic antioxidant. Thiobarbituric acid reactive substances ( TBARS), both basal and iron-stimulated, were significantly elevated in all vanadyl-treated animals. Vanadyl treatment lowered liver glutamine synthetase activities in diabetic rats, but not in non-diabetic animals. Thus, vanadyl treatment was antioxidant in terms of cataract formation and reduced glutathione concentration in liver homogenates, pro-oxidant by reason of iron-stimulated TBARS formation and inconclusive with respect to glutamine synthetase activity. These results highlight the importance of using multiple indicators of peroxidative change in evaluating new pro-oxidant/ antioxidant treatment regimens.
|
Authors | K H Thompson, J H McNeill |
Journal | Research communications in chemical pathology and pharmacology
(Res Commun Chem Pathol Pharmacol)
Vol. 80
Issue 2
Pg. 187-200
(May 1993)
ISSN: 0034-5164 [Print] United States |
PMID | 8100638
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Thiobarbituric Acid Reactive Substances
- Vanadium Compounds
- Vanadium
- Streptozocin
- vanadyl sulfate
- Glutamate-Ammonia Ligase
- Glutathione
|
Topics |
- Administration, Oral
- Animals
- Cataract
(metabolism)
- Diabetes Mellitus, Experimental
(metabolism)
- Glutamate-Ammonia Ligase
(metabolism)
- Glutathione
(metabolism)
- Lipid Peroxidation
(drug effects)
- Liver
(drug effects, enzymology)
- Male
- Rats
- Rats, Wistar
- Streptozocin
- Thiobarbituric Acid Reactive Substances
(metabolism)
- Vanadium
(metabolism, pharmacology)
- Vanadium Compounds
|