In vivo antimelanoma effects of 4-S-cysteaminylphenol, a newly synthesized therapeutic agent specific to melanoma.

Abstract

Antimelanoma effects of 4-S-cysteaminylphenol (4-S-CAP), which is a newly synthesized melanin precursor, as a chemotherapeutic agent specific to malignant melanoma were determined in an in vivo system using mouse B16 melanoma. The intraperitoneal injection of 4-S-CAP induced a slight delay in the growth period of subcutaneous melanoma of C57BL/6 mice. Survival times of mice after treatment with 4-S-CAP were a little longer than those of control mice (P < 0.05), although all mice died from the local growth of tumours. The viable cell ratio of in vivo subcutaneous tumour cells reduced to 52.8% within 24 h after treatment with 4-S-CAP, but the ratio had recovered to the control level 72 h after treatment (> 90%). Similarly, the proliferating-cell-nuclear-antigen-positive cell ratio of melanotic melanoma had reduced 24 h after treatment and recovered within 72 h after treatment, while 4-S-CAP had no effect on amelanotic tumours. The formation of lung colonies by intravenous inoculation of malignant melanoma cells was compared between mice with intraperitoneal injection of 4-S-CAP and phosphate-buffered saline only. The 4-S-CAP-treated mice had significantly fewer lung colonies compared with the control mice (P < 0.01). The results indicate that the agent, 4-S-CAP, would have a therapeutic effect on malignant melanoma for a short time in vivo and therefore the agent can be effective against a small number of tumour cells, such as lung colonies, although it had little effect on the local tumours.

• Authors: M Kitagawa, T Nemoto, S Seki, S Ito, T Kasuga
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 119 Issue 8 Pg. 470-4 ( 1993) ISSN: 0171-5216 [Print] Germany
• PMID: 8099588 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• Antineoplastic Agents
• Nuclear Proteins
• Proliferating Cell Nuclear Antigen
• Cysteamine
• 4-S-cysteaminylphenol

Topics

• Animals
• Antigens, Neoplasm (drug effects)
• Antineoplastic Agents (therapeutic use)
• Cell Cycle (drug effects)
• Cysteamine (analogs & derivatives, therapeutic use)
• Immunohistochemistry
• Lung Neoplasms (drug therapy)
• Male
• Melanoma, Experimental (chemistry, drug therapy, pathology)
• Mice
• Mice, Inbred C57BL
• Nuclear Proteins (drug effects)
• Proliferating Cell Nuclear Antigen