Comparison of the effects of tumour necrosis factor alpha stimulation and phorbol ester treatment on the immunocytochemical staining of intercellular adhesion molecule 1 in human renal carcinoma cell cultures.

Abstract

Incubation of the human renal carcinoma cell line CaKi-1 with tumour necrosis factor alpha (TNF alpha) or the phorbol ester phorbol-12-myristate 13 acetate (PMA) strongly enhanced the immunocytochemical staining of the intercellular adhesion molecule ICAM-1, in a non-linear manner. Since PMA is capable of activating Ca2+/phospholipid-dependent protein kinase C (PKC), we investigated the role of this kinase during TNF alpha signal transduction. Calcium ionophore A23187 significantly enhanced PMA, but not TNF alpha-induced ICAM-1 staining. The PKC inhibitors H7, staurosporine and sphingosine abrogated the action of PMA, while TNF alpha was unaffected. Simultaneous incubation with TNF alpha and PMA resulted in maximal ICAM-1 staining significantly above values obtained when cultures were treated with either agent alone. Finally, chronic PMA treatment with subsequent TNF alpha stimulation enhanced ICAM-1 staining above values from cultures where TNF alpha was omitted. Our findings suggest that the immunocytochemical staining of ICAM-1 in CaKi-1 cells can be induced by TNF alpha through mainly PKC-independent mechanisms or by PMA through PKC-dependent mechanisms. The two agents may work synergistically in this respect.

• Authors: A B Hansen, C B Andersen
• Journal: Virchows Archiv. B, Cell pathology including molecular pathology (Virchows Arch B Cell Pathol Incl Mol Pathol) Vol. 63 Issue 2 Pg. 107-13 ( 1993) ISSN: 0340-6075 [Print] Germany
• PMID: 8094920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Cell Adhesion Molecules
• Isoquinolines
• Piperazines
• Tumor Necrosis Factor-alpha
• Intercellular Adhesion Molecule-1
• Calcimycin
• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
• Protein Kinase C
• Staurosporine
• Sphingosine
• Tetradecanoylphorbol Acetate

Topics

• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
• Alkaloids (pharmacology)
• Calcimycin (pharmacology)
• Carcinoma, Renal Cell (chemistry)
• Cell Adhesion Molecules (analysis)
• Humans
• Immunoenzyme Techniques
• Intercellular Adhesion Molecule-1
• Isoquinolines (pharmacology)
• Kidney Neoplasms (chemistry)
• Piperazines (pharmacology)
• Protein Kinase C (antagonists & inhibitors)
• Signal Transduction (drug effects)
• Sphingosine (pharmacology)
• Staurosporine
• Tetradecanoylphorbol Acetate (pharmacology)
• Tumor Cells, Cultured
• Tumor Necrosis Factor-alpha (pharmacology)