Transamination and transsulphuration of L-cysteine in Ehrlich ascites tumor cells and mouse liver. The nonenzymatic reaction of L-cysteine with pyruvate.

Abstract

1. The activity of cysteine aminotransferase (CAT), 3-mercaptopyruvate sulfurtransferase (MPST) and rhodanese is much lower in Ehrlich ascites tumor cells (EATC) than in mouse liver. 2. Contrary to mouse liver homogenate, no synthesis of sulphane sulphur-containing compounds from L-cysteine is observed in EATC homogenate. 3. 2-Methyl-thiazolidine-2,4-dicarboxylic acid (CP), 2-methyl-thiazolidine-4-carboxylic acid (CA) and thiazolidine-4-carboxylic acid (CF) can be used as sources of low molecular-weight thiol compounds both in EATC and mouse liver homogenate. 4. Pyruvate formed from phosphoenolpyruvate (PEP) in EATC homogenates reacts with L-cysteine (L-CYS) to CP.

• Authors: L Włodek, M Wróbel, J Czubak
• Journal: The International journal of biochemistry (Int J Biochem) Vol. 25 Issue 1 Pg. 107-12 (Jan 1993) ISSN: 0020-711X [Print] England
• PMID: 8094343 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Amines
• Glutamates
• Thiocyanates
• Glutamic Acid
• Sulfur
• Transaminases
• cysteine aminotransferase
• Sulfurtransferases
• Thiosulfate Sulfurtransferase
• 3-mercaptopyruvate sulphurtransferase
• Cystathionine gamma-Lyase
• Cysteine
• thiocyanate

Topics

• Amines (metabolism)
• Animals
• Biotransformation
• Carcinoma, Ehrlich Tumor (enzymology, metabolism)
• Cystathionine gamma-Lyase (metabolism)
• Cysteine (metabolism)
• Glutamates (metabolism)
• Glutamic Acid
• Liver (enzymology, metabolism)
• Mice
• Sulfur (metabolism)
• Sulfurtransferases (metabolism)
• Thiocyanates (metabolism)
• Thiosulfate Sulfurtransferase (metabolism)
• Transaminases (metabolism)