Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.

Abstract

A cell line (SMKT-R3) established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic labelling with 35S-sulfate. These sulfolipids were expressed at the surface of SMKT-R3 cells as ascertained by cytofluorometry using a monoclonal antibody directed to sulfolipids. Furthermore, markedly high activity levels of glycolipid sulfotransferases were observed in SMKT-R3 cells compared with other cell lines. These results suggest that the increased synthesis of sulfolipids in renal cell carcinoma tissue (Sakakibara et al., 1989. Cancer Res., 49, 335-339) is due to the elevation of the sulfotransferase activities of renal carcinoma cells themselves.

• Authors: T Kobayashi, K Honke, K Kamio, N Sakakibara, S Gasa, N Miyao, T Tsukamoto, I Ishizuka, T Miyazaki, A Makita
• Journal: British journal of cancer (Br J Cancer) Vol. 67 Issue 1 Pg. 76-80 (Jan 1993) ISSN: 0007-0920 [Print] England
• PMID: 8094007 (Publication Type: Journal Article)

Chemical References

• Glycolipids
• Lipids
• sulfolipids
• Sulfurtransferases
• Sulfotransferases
• galactosylceramide sulfotransferase
• Cerebroside-Sulfatase

Topics

• Carcinoma, Renal Cell (enzymology, metabolism)
• Cerebroside-Sulfatase (metabolism)
• Flow Cytometry
• Glycolipids (metabolism)
• Humans
• Kidney Neoplasms (enzymology, metabolism)
• Lipid Metabolism
• Lipids
• Sulfotransferases
• Sulfurtransferases (metabolism)
• Tumor Cells, Cultured