Actin-binding protein, drebrin, accumulates in submembranous regions in parallel with neuronal differentiation.

Abstract

Drebrins are developmentally regulated actin-binding proteins. In this study, we analyzed subcellular distribution of drebrin E in neuroblastoma cells (SH-SY5Y) in culture, especially in terms of its relationship to actin filaments. In undifferentiated cells, drebrin E was scattered as flocculus small dots along the stress fibers and also accumulated at adhesion plaques. In parallel with the neuronal differentiation following retinoic acid treatment, drebrin E was accumulated, accompanying filamentous (F) actin, in the submembranous cortical cytoplasm. Similar submembranous localization of drebrins was observed in primary cultured neurons. In the presence of drebrin E F-actin was more stable against cytochalasin D than F-actin lacking drebrin E. These results suggest that drebrin E plays a role in neuronal morphological differentiation by changing its subcellular localization with stabilized F-actin.

• Authors: H Asada, K Uyemura, T Shirao
• Journal: Journal of neuroscience research (J Neurosci Res) Vol. 38 Issue 2 Pg. 149-59 (Jun 01 1994) ISSN: 0360-4012 [Print] United States
• PMID: 8078100 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Cytoskeletal Proteins
• Neuropeptides
• drebrins
• Cytochalasin D
• Tretinoin

Topics

• Actins (metabolism)
• Brain Neoplasms (metabolism)
• Cell Differentiation (physiology)
• Cytochalasin D (pharmacology)
• Cytoskeletal Proteins (metabolism)
• Electrophoresis, Polyacrylamide Gel
• Humans
• Immunoblotting
• Immunohistochemistry
• Neuroblastoma (metabolism)
• Neurons (drug effects, metabolism, physiology)
• Neuropeptides (biosynthesis, immunology, metabolism)
• Subcellular Fractions (drug effects, metabolism)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured