Immunoglobulins and alpha 1-acid glycoprotein do not contribute to the cholesterol crystallization-promoting effect of concanavalin A-binding biliary protein.
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| Abstract |
Human bile contains cholesterol crystallization-stimulating proteins that can be isolated by concanavalin A-Sepharose chromatography. In the past few years an increasing number of different pronucleating proteins have been identified in the concanavalin A-binding fraction. In this study we attempted to estimate the relative contribution of a number of these proteins to total concanavalin A-binding pronucleating activity. For this purpose, concanavalin A-binding glycoproteins were isolated from gallbladder bile samples from 12 patients with gallstones. The role of IgA, IgG and IgM and alpha 1-acid glycoprotein was investigated by means of immunoextraction. No decrease in crystallization-promoting activity was observed after precipitation of more than 98% of the different immunoglobulins. In addition, removal of more than 95% of alpha 1-acid glycoprotein from different concanavalin A-binding fractions had no significant effect on cholesterol crystallization-promoting activity. The influence of fibronectin was estimated by addition of physiological concentrations to a model bile system. At these concentrations fibronectin did not promote crystallization. From these data we conclude that immunoglobulins, alpha 1-acid glycoprotein and probably also fibronectin do not significantly contribute to total concanavalin A-binding activity.
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| Authors | M A de Bruijn, K S Mok, T Out, G N Tytgat, A K Groen |
| Journal | Hepatology (Baltimore, Md.) (Hepatology) Vol. 20 Issue 3 Pg. 626-32 (Sep 1994) ISSN: 0270-9139 [Print] United States |
| PMID | 8076920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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