Serum levels of steroid sulfatase protein in gynecologic carcinomas.

Abstract

Steroid sulfatase (STS) desulfates a number of 3 beta-hydroxysteroid sulfates, converting inactive steroid hormone to the active form. We have established an enzyme-linked immunosorbent assay (ELISA) of STS by using polyclonal antibody against STS purified from human placenta to measure the amount of the enzyme protein in sera. ELISA was performed by a 'Sandwich' method using a peroxidase conjugated anti-STS IgG Fab' fragment. A range of STS of 10-1,500 ng/ml in serum was assayed by this method. When the serum STS from the patients with gynecologic carcinomas was assayed by the ELISA, the level was significantly elevated in endometrial carcinoma (P < 0.05) and ovarian carcinoma (P < 0.01), respectively, as compared with that of normal healthy women.

• Authors: T Sugawara, K Honke, S Gasa, T Tanaka, S Fujimoto, A Makita
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 226 Issue 1 Pg. 13-20 (Apr 1994) ISSN: 0009-8981 [Print] Netherlands
• PMID: 8070131 (Publication Type: Journal Article)

Chemical References

• Immunoglobulin Fab Fragments
• Arylsulfatases
• Steryl-Sulfatase

Topics

• Adult
• Aged
• Aging (blood)
• Animals
• Arylsulfatases (blood, immunology, isolation & purification)
• Blotting, Western
• Carcinoma (enzymology)
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• Female
• Genital Neoplasms, Female (enzymology)
• Humans
• Immunoglobulin Fab Fragments
• Male
• Middle Aged
• Rabbits
• Reference Values
• Steryl-Sulfatase