Simultaneous determination of primaquine and carboxyprimaquine in plasma using high-performance liquid chromatography with electrochemical detection.

Abstract

A selective and sensitive high-performance liquid chromatographic method with electrochemical detection is described for the simultaneous quantitation of primaquine and carboxyprimaquine, its primary metabolite, in plasma. After addition of internal standard, plasma was deproteinized by addition of acetonitrile. Nitrogen-dried supernatants, resuspended in mobile phase were analyzed on a C8 reversed-phase column. Limits of detection for primaquine and carboxyprimaquine were 2 and 5 ng/ml with quantitation limits of 5 and 20 ng/ml, respectively. None of 47 tested antimicrobial agents interfered. In contrast to previously reported methods, the assay sensitivity and specificity are sufficient to permit quantitation of primaquine in plasma for pharmacokinetics following low dose (30 mg, base) oral administration of primaquine, typically used in the treatment of malaria and Pneumocystis carinii pneumonia.

• Authors: R A Dean, W Ochieng, J Black, S F Queener, M S Bartlett, N G Dumaual
• Journal: Journal of chromatography. B, Biomedical applications (J Chromatogr B Biomed Appl) Vol. 655 Issue 1 Pg. 89-96 (Apr 22 1994) ISSN: 1572-6495 [Print] Netherlands
• PMID: 8061837 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Drug Combinations
• Indicators and Reagents
• 8-(3-carboxy-1-methylpropylamino)-6-methoxyquinoline
• Primaquine

Topics

• Acquired Immunodeficiency Syndrome (drug therapy)
• Chromatography, High Pressure Liquid
• Drug Combinations
• Electrochemistry
• Humans
• Indicators and Reagents
• Primaquine (analogs & derivatives, blood, pharmacokinetics)