Production and characterization of new monoclonal antibodies that distinguish subsets of mink lymphoid cells.
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| Abstract |
Several hybridoma clones that produce monoclonal antibodies (MAbs) reacting with subpopulations of mink lymphoid cells were established. Two of the MAbs, MTS-4.3 and MTS-9.3, reacted with relatively small populations of surface immunoglobulin (Ig)-negative (Ig-) lymphocytes. MTS-4.3+ and MTS-9.3+ cells were distributed in the thymic cortex and medulla, paracortical areas of lymph nodes, and periarterial lymphoid sheaths of the spleen, indicating that these MAbs identify T lymphocytes. Another MAb, MTB-5.6, reacted with a large proportion of surface Ig+ lymph node cells, but not with surface Ig- cells. In immunohistochemistry this MAb stained dendritic epithelial cells of thymic cortex, large polygonal cells of thymic medulla, a large proportion of lymphocytes in the mantle zone of lymphoid follicles, dendritic-shaped cells of paracortical area, and some lymphocytes and macrophage-like cells of medullary cords and sinuses of lymph nodes. The expression of the cell-surface antigen reacting with MTB-5.6 on Ig+ lymph node cells was increased after concanavalin A stimulation. These new reagents may be useful to analyze cellular basis of the abnormal immune responses observed in Aleutian mink disease, a classical model of human autoimmune diseases.
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| Authors | M Miyazawa, S Mori, G J Spangrude, J B Wolfinbarger, M E Bloom |
| Journal | Hybridoma (Hybridoma) Vol. 13 Issue 2 Pg. 107-14 (Apr 1994) ISSN: 0272-457X [Print] United States |
| PMID | 8050775 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.) |
| Chemical References |
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