Human neuroblastoma growth inhibitory factor (h-NGIF), derived from human astrocytoma conditioned medium, has neurotrophic properties.

Abstract

Investigations on the general characteristics of human astrocytoma cell line NAC-1 revealed neuroblastoma growth inhibitory activity in conditioned medium. Neuroblastoma growth inhibitory factor (NGIF) was partially purified by Econo Q, Econo CM, and Superose 12 column chromatography. The protein is weakly basic with an estimated M(r) of 120,000, possibly having an M(r) 60,000 dimeric structure. NGIF inhibits the growth of human neuroblastoma cell lines but has no effect on morphology nor does it produce any change in the growth of human glioblastoma cell lines. Interestingly, NGIF appears to promote survival and neurite outgrowth of embryonal rat cortical neurons. These neurotrophic properties suggest a role for NGIF in the development of the nervous system.

• Authors: Y Z Eksioglu, J Iida, K Asai, T Ueki, K Nakanishi, I Isobe, K Yamagata, T Kato
• Journal: Brain research (Brain Res) Vol. 644 Issue 2 Pg. 282-90 (May 02 1994) ISSN: 0006-8993 [Print] Netherlands
• PMID: 8050039 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media, Conditioned
• Nerve Tissue Proteins
• Endopeptidases

Topics

• Animals
• Astrocytoma (metabolism)
• Cell Survival (drug effects)
• Cerebral Cortex (cytology, drug effects)
• Culture Media, Conditioned
• Endopeptidases
• Humans
• Hydrolysis
• Mice
• Molecular Weight
• Nerve Tissue Proteins (chemistry, isolation & purification, pharmacology)
• Nervous System Neoplasms (metabolism)
• Neuroglia (drug effects)
• Neurons (drug effects)
• Rats
• Tumor Cells, Cultured