Increased splanchnic prostacyclin synthase and cyclooxygenase content and activity during ischemia is due to new protein synthesis.

This study examines the hypothesis that the exaggerated splanchnic release of prostacyclin is due to new synthesis of both cyclooxygenase and prostacyclin synthase (PS) in the ileum muscularis/serosa.
Sprague-Dawley rats were anesthetized and subjected to acute hemorrhage to 30 mm Hg for 30 minutes (shock) or sham shock. The superior mesenteric artery (SMA) was cannulated and removed with its end-organ intestine and perfused in vitro with Krebs-Henseleit buffer with and without cycloheximide (50 micrograms/ml) or indomethacin (20 micrograms/ml). Venous effluent was analyzed for eicosanoids by radioimmunoassay. The SMA, aorta and ileal mucosa, and muscularis/serosa were analyzed for PS and cyclooxygenase content by immunoblot analysis.
The sham splanchnic bed released threefold more 6-keto-PGF1 alpha than prostaglandin E2 and thromboxane. Acute ischemia increased splanchnic release of 6-keto-PGF1 alpha threefold compared with sham, which was abolished by cycloheximide or indomethacin treatment. Acute ischemia increased content of PS and cyclooxygenase in the ileal muscularis/serosa twofold and PS in the aorta and SMA by 50%.
Acute ischemia increased release of 6-keto-PGF1 alpha, which was dependent on new protein synthesis. The immunoblot data suggest that the location of the increased enzymes responsible for increased 6-keto-PGF1 alpha release is the ileal muscularis/serosa and in the aorta and SMA.
AuthorsS I Myers, C T Evans, R Hernandez, L Bartula
JournalSurgery (Surgery) Vol. 116 Issue 2 Pg. 432-8 (Aug 1994) ISSN: 0039-6060 [Print] UNITED STATES
PMID8048009 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Eicosanoids
  • Cytochrome P-450 Enzyme System
  • Cycloheximide
  • Prostaglandin-Endoperoxide Synthases
  • Isomerases
  • Intramolecular Oxidoreductases
  • prostacyclin synthetase
  • Animals
  • Cycloheximide (pharmacology)
  • Cytochrome P-450 Enzyme System (biosynthesis)
  • Eicosanoids (biosynthesis)
  • Intramolecular Oxidoreductases
  • Ischemia (enzymology)
  • Isomerases (biosynthesis)
  • Mesentery (enzymology)
  • Prostaglandin-Endoperoxide Synthases (biosynthesis)
  • Rats
  • Rats, Sprague-Dawley

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: