Abstract |
Antibody IgG to human T-cell leukemia virus type I (HTLV-I) in urine was detected by a sensitive enzyme immunoassay ( immune complex transfer enzyme immunoassay) using a synthetic peptide, Cys-env gp46(188-224), as antigen, the sensitivity and specificity of which were 100 and 98.5%, respectively, using serum samples. Anti-HTLV-I IgG in urine was reacted simultaneously with 2,4-dinitrophenyl-bovine serum albumin- Cys-env gp46(188-224) conjugate and Cys-env gp46(188-224)-beta-D-galactosidase (Escherichia coli) conjugate. The complex formed, consisting of the three components, was trapped onto polystyrene balls coated with affinity-purified (anti-2,4-dinitrophenyl group) IgG, eluted with epsilon N-2,4-dinitrophenyl-L-lysine and transferred to polystyrene balls coated with affinity-purified (anti-human IgG gamma-chain) IgG. Finally, bound beta-D-galactosidase activity was assayed by fluorometry. Thirty-one urine samples from seropositive subjects and 100 urine samples from seronegative subjects were tested. The sensitivity and specificity were 87 and 100%, respectively, with unconcentrated urine samples and 94 and 100%, respectively, with approximately 10-fold concentrated urine samples. These results were superior to those by the conventional ELISA and gelatin particle agglutination test.
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Authors | S Hashida, K Hirota, T Kohno, E Ishikawa |
Journal | Journal of clinical laboratory analysis
(J Clin Lab Anal)
Vol. 8
Issue 3
Pg. 149-56
( 1994)
ISSN: 0887-8013 [Print] United States |
PMID | 8046543
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigen-Antibody Complex
- HTLV-I Antibodies
- HTLV-I Antigens
- Immunoglobulin G
- Peptide Fragments
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Topics |
- Adult
- Aged
- Amino Acid Sequence
- Animals
- Antigen-Antibody Complex
(metabolism)
- Female
- HTLV-I Antibodies
(urine)
- HTLV-I Antigens
(immunology)
- Humans
- Immunoenzyme Techniques
- Immunoglobulin G
(urine)
- Male
- Middle Aged
- Molecular Sequence Data
- Peptide Fragments
(immunology)
- Rabbits
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