[Function and amount of GTP binding proteins in postmortem human brain in dementia of the Alzheimer's type].

Abstract

Qualitative or quantitative alterations of GTP binding (G) proteins in membrane preparations from parietal cortex and temporal cortex in postmortem human brain obtained from patients with dementia of the Alzheimer's type (DAT) and aged and postmortem delay matched controls were investigated by immunoblotting with polyclonal antibodies (RM/1, AS/7, GC/2, QL, SW/1) against specific G protein (Gs alpha, Gi alpha, Go alpha, Gq alpha, G beta, respectively) and the functional photoaffinity GTP analog [azidoanilido GTP (AAGTP)] labeling. Although the quantitative immunoreactivity showed no significant differences between the two groups, a marked reduction in AAGTP binding to Gs alpha was seen in the DAT group compared with controls in both parietal and temporal cortex. Moreover, the ratio of Gs alpha to Gi/o alpha AAGTP binding decreased significantly in the same regions of the DAT group. These results suggest that the disturbances of G protein mediated signal transduction may be involved in the pathophysiology of DAT.

• Authors: E Hashimoto, H Ozawa, T Saito
• Journal: Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology (Nihon Shinkei Seishin Yakurigaku Zasshi) Vol. 14 Issue 2 Pg. 105-9 (Apr 1994) ISSN: 1340-2544 [Print] Japan
• PMID: 8042344 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• GTP-Binding Proteins

Topics

• Alzheimer Disease (metabolism)
• Brain (metabolism)
• GTP-Binding Proteins (metabolism, physiology)
• Humans
• Postmortem Changes
• Protein Binding
• Signal Transduction