Abstract |
We investigated the formation of F2-isoprostanes produced by non-enzymatic peroxidation of arachidonic acid during rabbit aortic endothelial cell-mediated oxidation of low density lipoprotein ( LDL). Free and total (sum of free and esterified) levels of F2-isoprostanes were measured using a solid-phase extraction procedure and gas chromatography-mass spectrometry. Free levels of F2-isoprostanes in native LDL were 0.06 +/- 0.03 ng/mg protein (n = 4), whereas total levels were 0.28 +/- 0.09 ng/mg protein (n = 4). Both free and total levels of the isoprostanes were found to increase during the oxidation. 8-epi-PGF2 alpha was the major isoprostane formed (free and total concentrations after 24 h, 2.50 +/- 0.24 and 6.42 +/- 1.36 ng/mg protein (n = 4), respectively). The release of F2-isoprostanes during aortic endothelial cell-induced oxidation of LDL could be a contributory factor in the development of atherosclerosis.
|
Authors | N K Gopaul, J Nourooz-Zadeh, A I Mallet, E E Anggård |
Journal | FEBS letters
(FEBS Lett)
Vol. 348
Issue 3
Pg. 297-300
(Jul 18 1994)
ISSN: 0014-5793 [Print] England |
PMID | 8034058
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Lipoproteins, LDL
- Thiobarbituric Acid Reactive Substances
- Dinoprost
|
Topics |
- Animals
- Aorta
(metabolism)
- Cell Survival
- Dinoprost
(biosynthesis)
- Endothelium, Vascular
(metabolism)
- Kinetics
- Lipid Peroxidation
- Lipoproteins, LDL
(metabolism)
- Oxidation-Reduction
- Rabbits
- Thiobarbituric Acid Reactive Substances
(metabolism)
|