Immunohistochemical analysis of CDw52 antigen expression in non-Hodgkin's lymphomas.

Abstract

AIM:

To determine the antigen expression of CDw52 using Campath-1 antibodies in a series of non-Hodgkin's lymphomas (NHLs).

METHODS:

Tissue sections of lymphoma were stained immunohistochemically using rat Campath-1G and humanised Campath-1H with avidin-biotin-peroxidase complex techniques. Fifty-two fresh frozen lymphomas and a further 26 paraffin wax embedded sections were studied.

RESULTS:

Thirty-seven out of 41 B cell lymphomas were positive with Campath-1H in frozen sections (low grade, 24 of 24; high grade, 13 of 17) as were three out of five T cell lymphomas. Reed-Sternberg cells in six cases of Hodgkin's disease did not react. Eleven out of 16 high grade B cell lymphomas also stained positively with Campath-1G in paraffin wax sections as did five out of 10 T cell lymphomas.

CONCLUSIONS:

The Campath-1 antibodies showed that CDw52 antigen expression was present in all cases of low grade B cell NHL examined. Immunohistochemical staining in high grade B cell NHL and in T cell NHL was variable. These findings may be relevant to patient selection when considering treatment with Campath-1 antibodies.

• Authors: J R Salisbury, N T Rapson, J D Codd, M V Rogers, A B Nethersell
• Journal: Journal of clinical pathology (J Clin Pathol) Vol. 47 Issue 4 Pg. 313-7 (Apr 1994) ISSN: 0021-9746 [Print] England
• PMID: 8027367 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• Antigens, Neoplasm
• CD52 Antigen
• CD52 protein, human
• Cd52 protein, rat
• Glycoproteins

Topics

• Antigens, CD (analysis)
• Antigens, Neoplasm
• CD52 Antigen
• Glycoproteins
• Humans
• Immunoenzyme Techniques
• Lymphoma, B-Cell (immunology)
• Lymphoma, Non-Hodgkin (immunology)
• Lymphoma, T-Cell (immunology)