[A novel method for studying the free radical status of phagocytotic blood cells in patients with hemoglobinopathies].

Abstract

Vital fluorochrome hydroethidine was used for measuring relative extra- and intracellular production of active oxygen in phagocytotic blood cells in hemoglobinopathies. Hydroethidine sensitivity to diverse free oxygen forms was studied in various model systems. Probability of false-positive results was estimated. It was established that hydroethidine interacts with superoxide anion radicals in molar ratio 1:1, that intracellular production of active oxygen forms in leukocytic mass cells stimulated by phorbol myristate acetate was enhanced in patients with major beta(+)-thalassemia and in a patient with hemoglobinopathy due to unstable hemoglobin Hb-Alesha. In thalassemia minor no significant differences compared to control were reported. Potential causes and value of the results obtained are under discussion.

• Authors: A V Biziukin, N S Smetanina, L G Korkina
• Journal: Gematologiia i transfuziologiia (Gematol Transfuziol) 1994 Mar-Apr Vol. 39 Issue 2 Pg. 41-6 ISSN: 0234-5730 [Print] Russia (Federation)
• Vernacular Title: Novyĭ metod izucheniia svobodnoradikal'nogo statusa fagotsitiruiushchikh kletok krovi u patsientov s gemoglobinopatiiami.
• PMID: 8026690 (Publication Type: English Abstract, Journal Article)

Chemical References

• Free Radicals
• Reactive Oxygen Species

Topics

• Adolescent
• Child
• Child, Preschool
• Free Radicals
• Hemoglobinopathies (blood)
• Humans
• Infant
• Phagocytosis (physiology)
• Reactive Oxygen Species