Crosslinking and cleavage of pBR322 DNA photosensitized by 7-methylpyrido[3,4-c]psoralen.

Abstract

7-Methylpyrido[3,4-c]psoralen (7-MPP) had been designed to be a monofunctional sensitizer in which the pyrone double bond, being engaged in a pyridine ring, would be incapable of participating in the formation of a cyclobutane ring with a DNA component. However, one example of photosensitization of thymine-thymine dimer formation in DNA by 7-MPP had been reported. This paper proves that 7-MPP can sensitize interstrand crosslinks in pBR322 DNA. It also proves that 7-MPP photosensitizes double strand cleavage reactions in the DNA with an unusually large degree of site selectivity.

• Authors: X Chen, J Kagan
• Journal: Journal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 23 Issue 1 Pg. 27-33 (Apr 1994) ISSN: 1011-1344 [Print] Switzerland
• PMID: 8021747 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• DNA, Bacterial
• Furocoumarins
• Photosensitizing Agents
• 7-methylpyrido(4,3-c)psoralen

Topics

• Carcinogens (pharmacology)
• DNA, Bacterial (drug effects, radiation effects)
• Escherichia coli
• Furocoumarins (pharmacology)
• Molecular Structure
• Photosensitizing Agents (pharmacology)
• Plasmids (drug effects, radiation effects)
• Ultraviolet Rays