In vitro effect of KCA-098, a derivative of coumestrol, on bone resorption of fetal rat femurs.

Abstract

The effects of 3,9-bis(N,N-dimethylcarbamoyloxy)-5H-benzofuro[3,2-c]quinoli ne-6-one (KCA-098), a derivative of coumestrol, on bone resorption was studied in organ cultures of 20-day fetal rat femora. KCA-098 increased the length, dry weight, and calcium and phosphorus contents of parathyroid hormone (PTH)-treated fetal rat femur. As PTH significantly reduced the calcium and phosphorus contents of the femora, probably by stimulating bone resorption, KCA-098 seems to inhibit bone resorption. In fact, KCA-098 inhibited the PTH-induced release of 45Ca from pre-labeled fetal rat femora into the medium in organ culture. Coumestrol also inhibited the release of 45Ca from bone into the medium. However, KCA-098 did not increase the uterine weight of ovariectomized rats, whereas coumestrol did so. Thus KCA-098 is a unique, new inhibitor of bone resorption that has no estrogenic activity.

• Authors: N Tsutsumi, K Kawashima, N Arai, H Nagata, M Kojima, A Ujiie, H Endo
• Journal: Bone and mineral (Bone Miner) Vol. 24 Issue 3 Pg. 201-9 (Mar 1994) ISSN: 0169-6009 [Print] Ireland
• PMID: 8019207 (Publication Type: Journal Article)

Chemical References

• Parathyroid Hormone
• KCA 098
• Phosphorus
• Estradiol
• Calcium
• Coumestrol

Topics

• Animals
• Bone Resorption (embryology)
• Calcification, Physiologic
• Calcium (metabolism)
• Coumestrol (analogs & derivatives, pharmacology)
• Estradiol (pharmacology)
• Female
• Femur (embryology, physiology)
• Organ Culture Techniques
• Organ Size (drug effects)
• Ovariectomy
• Parathyroid Hormone (pharmacology)
• Phosphorus (metabolism)
• Pregnancy
• Rats
• Rats, Wistar
• Uterus (anatomy & histology)