Activity of diclazuril against Toxoplasma gondii in cultured cells and mice.

Abstract

The capability of diclazuril, a benzeneacetonitrile anticoccidial agent, to inhibit development of tachyzoites of Toxoplasma gondii was examined in cultured human foreskin fibroblast cells and in Hsd:ICR mice. Treatment of infected cell cultures with 10.0, 1.0, 0.1 or 0.01 microgram of diclazuril/ml for 3 days resulted in > 99% reduction in tachyzoite counts, compared with controls. Treatment with 0.005 microgram of diclazuril/ml resulted in > 97% reduction in tachyzoite counts, compared with controls. Treatment of host cells with 10.0, 1.0, 0.1 and 0.01 microgram of diclazuril/ml for 24 hours prior to tachyzoite inoculation resulted in 97, 31, 0, and 0% reduction in tachyzoite counts, compared with controls, respectively, 3 days after inoculation. All mice that were treated orally with 10.0 mg of diclazuril/kg of body weight and 80% of mice treated orally with 1.0 mg of diclazuril/kg 1 day prior to and for 10 days after tachyzoite inoculation were protected against acute toxoplasmosis. Mice treated with 10.0 mg of diclazuril/kg did not develop protective immunity, whereas mice treated with 1.0 mg of diclazuril/kg survived challenge exposure.

• Authors: D S Lindsay, B L Blagburn
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 55 Issue 4 Pg. 530-3 (Apr 1994) ISSN: 0002-9645 [Print] United States
• PMID: 8017699 (Publication Type: Journal Article)

Chemical References

• Nitriles
• Triazines
• diclazuril

Topics

• Animals
• Cells, Cultured
• Female
• Fibroblasts (cytology)
• Humans
• Mice
• Mice, Inbred ICR
• Nitriles (pharmacology)
• Toxoplasma (drug effects)
• Toxoplasmosis, Animal (drug therapy)
• Triazines (pharmacology)