| Abstract | In order to clarify the autoimmune mechanisms of anti-Tg antibody (anti-TgAb) in autoimmune thyroid disease (AITD), a series of examinations were conducted in patients with Graves' disease (n = 59), Hashimoto's thyroiditis (n = 63) and healthy controls (n = 38). Our findings can be summarized as follows: 1) The distribution of anti-TgAb was measured by IgG subclass ELISA. IgG1 and/or IgG4 antibodies formed the major anti-Tg response, but in some patients, IgG2 anti-Tg tend to be the predominant response. We used ELISA to determine an IgG1 anti-Tg bound to Tg that had already interacted with IgG2 Tg-antibody (and vice versa). The results substantiated the view that TgAb of a different IgG subclass interact with different epitopes on Tg. 2) Human antiidiotypic (anti-Id) antibodies to anti-Tg antibody occur spontaneously in the AITD. These anti-Id antibodies can be divided into two categories based on properties of their binding sites. One type acts like a 'internal image' of Tg antigen which shown Ab2 beta activity. Another type has Ab2 alpha activity that recognizes Id determinants in the framework region common among anti-Tg antibody. 3) We also examined the competitive binding assay between TgAb and TgAb F(ab')2 fragments, and demonstrated differences in the TgAb repertoires between patients. 4) Soluble IL-2 receptor (IL-2R) of patients significantly increased as compared with normal subjects and IL-2R values in GD were higher than those in HT (p < 0.001). Therefore, IL-2R is regarded as a useful marker for disease activity. |
| Authors | L L Xiu
(Affiliation: Department of Clinical Laboratory Medicine, Hiroshima University School of Medicine, Japan.)
|
| Journal | Hiroshima journal of medical sciences
(Hiroshima J Med Sci)
Vol. 43
Issue 1
Pg. 1-11
(Mar 1994)
ISSN: 0018-2052 JAPAN |
| PMID | 8014070
(Publication Type: Journal Article)
|
| Chemical References |
- Antibodies
- Antibodies, Anti-Idiotypic
- Immunoglobulin G
- Receptors, Interleukin-2
- Thyroglobulin
|
| Topics |
- Adult
- Aged
- Antibodies
(blood)
- Antibodies, Anti-Idiotypic
(blood)
- Female
- Graves Disease
(immunology)
- Humans
- Immunoglobulin G
(blood)
- Male
- Middle Aged
- Receptors, Interleukin-2
(analysis)
- Thyroglobulin
(immunology)
- Thyroiditis, Autoimmune
(immunology)
|
