[Effect of targeting treatment of mitomycin C immunoconjugate on stomach neoplasm]

• Abstract: An anti-gastric cancer monoclonal antibody MGb2-mitomycin C conjugate via dextran T-70 as intermediate (MGb2-PAD-MMC) was produced, and 28-30 g molecules of MMC were introduced into 1 g molecule of MGb2. Ninety-six hours after ip of 125I-MGb2-PAD-MMC (1.48 MBq/22 micrograms MGb2 per mouse) to nude mice bearing human gastric cancer SGC-7901, the tumor tissue:blood (T/NT) radioactivity ratio was 2.6, very much higher than that of the control 125I-normal IgG-PAD-MMC group (T/NT = 0.20). Single photo computed tomography imaging confirmed the results of biodistribution study. MGb2-PAD-MMC exhibited selective killing action on the SGC-7901 cells in vitro, which was considered to be mediated by monoclonal antibody MGb2. Nude mice inoculated with SGC-7901 xenograft in bilateral subrenal capsule were treated by MMC (ip), human recombinant interferon-alpha (Hu-IFN-alpha im), MGb2-PAD-MMC (ip) and MGb2-PAD-MMC+Hu-IFN-alpha daily for 5 d beginning 4 h after inoculation. The efficacy of the reagents estimated by the reduction of tumor size and calculated by T/C (%), was 28.3%, 16.4%, 47.8%, and 83.1%, respectively. These results demonstrated that the antitumor effect of MGb2-PAD-MMC was superior to free MMC, and that the Hu-IFN-alpha might further enhance the action of MGb2-PAD-MMC.
• Authors: L J Chen, S Y Zhang, J L Zhang, Z Zhang, S Li, M Chen, X Y Zhang, X T Chen (Affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences.)
• Journal: Zhongguo yao li xue bao = Acta pharmacologica Sinica (Zhongguo Yao Li Xue Bao) Vol. 14 Issue 6 Pg. 572-6 (Nov 1993) ISSN: 0253-9756 [Print] CHINA
• PMID: 8010062 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Immunotoxins
• Interferon Type I, Recombinant
• Mitomycin

Topics

• Adenocarcinoma (pathology, therapy)
• Animals
• Antibodies, Monoclonal
• Humans
• Immunotoxins (therapeutic use)
• Interferon Type I, Recombinant (therapeutic use)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Mitomycin (therapeutic use)
• Stomach Neoplasms (pathology, therapy)
• Subrenal Capsule Assay
• Tumor Cells, Cultured