Abstract |
The T cell response to different Leishmania donovani antigens was investigated using peripheral blood mononuclear cells (PBMC) from Kenyans cured of visceral leishmaniasis and non-exposed Danes. Crude promastigote and amastigote antigens both induced proliferation and interferon-gamma (IFN-gamma) production in PBMC from cured patients, while cells from non-exposed donors gave weak responses. A similar pattern was induced by lipophosphoglycan-associated protein (LPGAP). By contrast, the major surface protease of Leishmania, gp63, induced only a weak proliferative response without IFN-gamma production in five of 17 samples from cured patients. Four of the five responding cultures produced IL-4, i.e. the response to this antigen was of the Th2 type. Furthermore, sera from acutely ill visceral leishmaniasis patients contained high levels of IgG antibodies to gp63. The Th2-like response to gp63 in patients cured of visceral leishmaniasis differs from the Th1-like response to the same antigen observed in patients cured of cutaneous leishmaniasis.
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Authors | J A Kurtzhals, A S Hey, A Jardim, M Kemp, K U Schaefer, E O Odera, C B Christensen, J I Githure, R W Olafson, T G Theander |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 96
Issue 3
Pg. 416-21
(Jun 1994)
ISSN: 0009-9104 [Print] England |
PMID | 8004810
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Protozoan
- Antigens, Protozoan
- Glycosphingolipids
- Protozoan Proteins
- lipophosphonoglycan
- Interleukin-4
- Interferon-gamma
- Metalloendopeptidases
- glycoprotein gp63, Leishmania
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Topics |
- Animals
- Antibodies, Protozoan
(blood)
- Antigens, Protozoan
- Glycosphingolipids
(immunology)
- Humans
- In Vitro Techniques
- Interferon-gamma
(biosynthesis)
- Interleukin-4
(biosynthesis)
- Leishmania donovani
(immunology)
- Leishmaniasis, Visceral
(immunology)
- Lymphocyte Activation
- Metalloendopeptidases
(immunology)
- Protozoan Proteins
(immunology)
- T-Lymphocyte Subsets
(immunology)
- T-Lymphocytes
(immunology)
- T-Lymphocytes, Helper-Inducer
(immunology)
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