Abstract |
We report the development of a monoclonal antibody-based enzyme immunoassay (EIA) specific for human urinary 18-hydroxycortisol, a biological marker of primary aldosteronism. Hybrid cell lines (hybridomas) were isolated after fusion between myeloma cells and spleen cells prepared from mice immunized with 18-hydroxycortisol conjugate. A competitive EIA suitable for the measurement of urinary 18-hydroxycortisol was developed using the mouse monoclonal antibody, KTM-41, which showed no practical cross-reaction with related endogenous steroids and synthetic steroids. This EIA meets all the requirements of routine clinical assay in terms of sensitivity (detection limit: 20 nmol/L), reproducibility (total CV: 8-15%), accuracy (recovery: 88-115%), simplicity and rapidity (< 3 h). Urinary 18-hydroxycortisol measured by the present assay was 153 +/- 119 nmol/L (mean +/- SD, range, 28-485) and 1787 +/- 1180 (range, 810-4264) in normal subjects (n = 20) and in patients with primary aldosteronism (n = 7), respectively. Clinical validation of the assay was confirmed by an appropriate decrease in urinary 18-hydroxycortisol level in patients with primary aldosteronism subsequent to adrenalectomy: 171 +/- 141 nmol/L (range, 41-466).
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Authors | H Kohno, S Sato, H Chiba, K Kobayashi, S Ikegawa, T Kurosawa, M Tohma |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 27
Issue 4
Pg. 277-82
(Aug 1994)
ISSN: 0009-9120 [Print] United States |
PMID | 8001289
(Publication Type: Clinical Trial, Comparative Study, Journal Article, Randomized Controlled Trial)
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Chemical References |
- Antibodies, Monoclonal
- Biomarkers
- 18-hydroxycortisol
- Hydrocortisone
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Antibody Specificity
- Binding, Competitive
- Biomarkers
- Calibration
- Chromatography, Affinity
- Cross Reactions
- Humans
- Hybridomas
- Hydrocortisone
(analogs & derivatives, immunology, urine)
- Hyperaldosteronism
(diagnosis, urine)
- Immunoenzyme Techniques
- Male
- Mice
- Mice, Inbred BALB C
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