Autoradiographic determination of regional cerebral blood flow and metabolism in conscious rats after fluid resuscitation from haemorrhage with a haemoglobin-based oxygen carrier.

Abstract	The effects of resuscitation fluids on the brain have been investigated in previous studies by global measurements of cerebral blood flow and metabolism. In this study we have examined the effects of a novel haemoglobin-based oxygen carrier on local cerebral blood flow (LCBF) and local cerebral glucose utilization (LCGU) after resuscitation from a volume-controlled haemorrhage of 30 min (3.0 ml/100 g body weight) with ultrapurified, polymerized, bovine haemoglobin (UPBHB). LCBF and LCGU were measured in 34 brain structures of conscious rats 2 h after resuscitation using quantitative iodo(14C)antipyrine and 2-(14C)-deoxy-D-glucose methods. The data were compared with a control group without haemorrhage and fluid resuscitation. In the haemorrhage group, LCBF increased after resuscitation by 12-56% in the different brain structures (mean 36%). LCGU changed less (0 to +18%, mean +9%). In the control group there was a close relationship between LCGU and LCBF (r = 0.95). After fluid resuscitation the relationship was preserved (r = 0.95), although it was reset at a higher ratio of LCBF to LCGU (P < 0.05). We conclude that fluid resuscitation of a 30 min volume-controlled haemorrhage using the haemoglobin-based oxygen carrier, UPBHB, induced a moderate degree of heterogeneity in the resulting changes of LCGU and LCBF. Local disturbances of cerebral blood flow or metabolism were not observed.
Authors	K F Waschke, D M Albrecht, K van Ackern, W Kuschinsky
Journal	British journal of anaesthesia (Br J Anaesth) Vol. 73 Issue 4 Pg. 522-8 (Oct 1994) ISSN: 0007-0912 [Print] England
PMID	7999496 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Blood Glucose Blood Substitutes Oxygen
Topics	Animals Autoradiography Blood Glucose (metabolism) Blood Pressure (physiology) Blood Substitutes (therapeutic use) Brain (metabolism) Cerebrovascular Circulation (physiology) Hemorrhage (physiopathology, therapy) Male Oxygen (blood) Partial Pressure Rats Rats, Sprague-Dawley

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