Abstract |
Annexin V is a human phospholipid binding protein (M(r) 36,000) that binds with high affinity to activated platelets in vitro. We studied the biodistribution and thrombus binding of annexin V in rabbit and swine models of fully occlusive arterial thrombi formed 1-2 h prior to injection of annexin V. Iodinated annexin V was cleared from blood in a rapid early phase (t1/2 = 6.4 min, 76% of radioactivity) and a slower late phase (t1/2 = 71 min, 24% of radioactivity). Organ uptake was highest in the kidney and spleen and lowest in heart and skeletal muscle. Thrombus/blood uptake ratios were (mean +/- SEM): 6.39 +/- 1.80 for rabbit iliac artery, 6.97 +/- 1.45 for swine carotid artery, and 7.68 +/- 1.70 for swine femoral artery (all p values < 0.01 versus control artery); a control protein, ovalbumin, showed an uptake ratio of 0.59 +/- 0.08 in swine femoral artery thrombi. These results indicate that annexin V is useful as an agent for selective targeting of platelet-containing thrombi.
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Authors | J F Tait, M D Cerqueira, T A Dewhurst, K Fujikawa, J L Ritchie, J R Stratton |
Journal | Thrombosis research
(Thromb Res)
Vol. 75
Issue 5
Pg. 491-501
(Sep 01 1994)
ISSN: 0049-3848 [Print] United States |
PMID | 7992250
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Annexin A5
- Iodine Radioisotopes
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Topics |
- Animals
- Annexin A5
(blood, pharmacokinetics, pharmacology)
- Biological Availability
- Biological Transport
(physiology)
- Blood Platelets
(drug effects, metabolism, physiology)
- Carotid Arteries
(metabolism, pathology, physiology)
- Carotid Artery Thrombosis
(metabolism, pathology, physiopathology)
- Disease Models, Animal
- Female
- Iliac Artery
(embryology, pathology, physiology)
- Iodine Radioisotopes
- Kidney
(embryology, pathology, physiology)
- Male
- Muscle, Skeletal
(metabolism, pathology, physiology)
- Rabbits
- Spleen
(metabolism, pathology, physiology)
- Swine
- Thrombosis
(blood, metabolism, physiopathology)
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