Genotyping of Canadian hepatitis C virus isolates by PCR.

Abstract

We used PCR for hepatitis C virus (HCV) genotyping with type-specific primers from the core and NS5 genes. Type I was predominant in the general population (58% in blood donors) as well as in different risk groups, such as intravenous drug abusers (58%), blood transfusion recipients (64%), hemophiliacs (62%), and patients with HCV chronic liver disease (76%). Types II, III, and IV were less prevalent in Canada, being found in 10.92, 6.72, and 5.88% of the population, respectively. The type II core primer was not type specific and reacted with the majority of our type I HCV samples, suggesting a false-positive dual infection with two different genotypes (I and II). Digestion of these amplified type I and type II products with restriction endonuclease AccI proved to be very useful in the exclusion of false-positive dual type I and type II infections.

• Authors: A Andonov, R K Chaudhary
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 32 Issue 8 Pg. 2031-4 (Aug 1994) ISSN: 0095-1137 [Print] United States
• PMID: 7989565 (Publication Type: Comparative Study, Journal Article)

Chemical References

• DNA Primers
• DNA, Viral
• Deoxyribonucleases, Type II Site-Specific
• GTMKAC-specific type II deoxyribonucleases

Topics

• Base Sequence
• Canada (epidemiology)
• DNA Primers
• DNA, Viral (genetics)
• Deoxyribonucleases, Type II Site-Specific (metabolism)
• Genotype
• Hepacivirus (classification, genetics)
• Hepatitis C (blood, epidemiology)
• Humans
• Molecular Sequence Data
• Polymerase Chain Reaction (methods)
• Risk Factors