Immune responses and protective effect in mice vaccinated orally with surface sporozoite protein of Eimeria falciformis in ISCOMs.

Abstract

Immunostimulating complexes (ISCOMs) were built after treatment of a purified surface protein from Eimeria falciformis sporozoites with a palmitic acid derivation, leading to a high ratio (33-64%) of P27 incorporation in these cage-like structures. P27 kept its antigenicity after incorporation in ISCOMs, which induced, after iterative intubations by the oral route to groups of mice, a systemic IgG response, a local IgA response, and a local enhanced cellular response as demonstrated by lymphoproliferation of mesenteric lymph node cells upon in vitro stimulation with antigen. This immunization (120 micrograms in six oral doses at 2-day intervals) afforded mice a partial protection (60%) against a subsequent 400 oocyst challenge. The reduction in daily oocyst excretion was corroborated by significantly different weight losses between immunized and control mice on days 9 and 10 postinfection and the subsequent death of these control mice. These observations provide the first application of ISCOMs to parasitic intestinal diseases.

• Authors: M Kazanji, F Laurent, P Péry
• Journal: Vaccine (Vaccine) Vol. 12 Issue 9 Pg. 798-804 (Jul 1994) ISSN: 0264-410X [Print] Netherlands
• PMID: 7975858 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Protozoan
• Antigens, Protozoan
• ISCOMs
• Membrane Proteins
• Palmitates
• Protozoan Proteins
• Protozoan Vaccines
• Vaccines, Attenuated

Topics

• Administration, Oral
• Animals
• Antibodies, Protozoan (biosynthesis)
• Antigens, Protozoan (genetics, immunology, metabolism)
• Blood Chemical Analysis
• Blotting, Western
• Cell Division (immunology)
• Cells, Cultured
• Coccidiosis (prevention & control)
• Eimeria (immunology)
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• ISCOMs (administration & dosage, immunology, therapeutic use, ultrastructure)
• Intestinal Mucosa (metabolism)
• Lymphocyte Activation (immunology)
• Male
• Membrane Proteins (immunology, metabolism)
• Mice
• Mice, Inbred BALB C
• Microscopy, Electron
• Palmitates (metabolism)
• Protozoan Proteins (immunology, metabolism)
• Protozoan Vaccines (administration & dosage, immunology, metabolism)
• Vaccination
• Vaccines, Attenuated (administration & dosage, immunology, metabolism)