Stimulation of intercellular adhesion molecule-1 (ICAM-1) antigen expression and shedding by interferon-gamma and phorbol ester in human renal carcinoma cell cultures: relation to peripheral blood mononuclear cell adhesion.

Abstract

In the present study we investigated the effect of interferon-gamma (IFN-gamma) and phorbol-12-myristate 13 acetate (PMA) on intercellular adhesion molecule-1 (ICAM-1) antigen expression and shedding in human renal carcinoma cell cultures. We also examined the functional consequences of ICAM-1 antigen expression and soluble ICAM-1 molecules on the adhesion of peripheral blood mononuclear cells (PBMC). Incubation of the human renal carcinoma cell line CaKi-1 with IFN-gamma or PMA enhanced ICAM-1 antigen expression. The calcium ionophore, 4-bromo-calcium ionophore A23187 (Bromo-A23187) significantly enhanced the IFN-gamma and PMA effect. Soluble ICAM-1 (sICAM-1) was detected in the supernatants of stimulated but not unstimulated cultures, and correlated significantly with cellular expression. Using 51Cr-labelled peripheral blood mononuclear cells in a cell adhesion assay, we demonstrated increased adhesion in IFN-gamma-treated CaKi-1 cultures, which was augmented by Bromo-A23187. This adhesion was blocked by preincubation of CaKi-1 cells with monoclonal antibody against ICAM-1 or by preincubation of PBMC with either monoclonal antibody against leucocyte function associated antigen-1 alpha (LFA-1 alpha), a major receptor for ICAM-1, supernatants from treated cultures or purified sICAM-1 molecules. Thus, shedding of ICAM-1 may play a role during the escape from immunosurveillance by renal carcinoma cells.

• Authors: A B Hansen, S T Lillevang, C B Andersen
• Journal: Urological research (Urol Res) Vol. 22 Issue 2 Pg. 85-91 ( 1994) ISSN: 0300-5623 [Print] Germany
• PMID: 7974918 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Lymphocyte Function-Associated Antigen-1
• Intercellular Adhesion Molecule-1
• Calcimycin
• 4-bromo-A-23187
• Interferon-gamma
• Tetradecanoylphorbol Acetate

Topics

• Antigens, Neoplasm (biosynthesis, drug effects)
• Calcimycin (analogs & derivatives, pharmacology)
• Carcinoma, Renal Cell (immunology)
• Cell Adhesion (drug effects)
• Humans
• Intercellular Adhesion Molecule-1 (immunology, physiology)
• Interferon-gamma (pharmacology)
• Kidney Neoplasms (immunology)
• Leukocytes, Mononuclear (cytology, drug effects)
• Lymphocyte Function-Associated Antigen-1 (physiology)
• Tetradecanoylphorbol Acetate (pharmacology)
• Tumor Cells, Cultured