Abstract | BACKGROUND: EXPERIMENTAL DESIGN: Kidney sections were exposed to exogenous bFGF and then to a monoclonal anti-bFGF antibody. Specificity of the interaction between HS and bFGF was established by monitoring concomitant loss of bFGF during selective removal of HS with heparitinase and competitive inhibition studies. To further characterize regional changes in saccharide sequences, heparitinase-generated unsaturated disaccharides, N-sulfated glucosamine-enriched but O- sulfate-scarce portions characteristics of native HS, and such portions characteristic of Engelbreth-Holm-Swarm tumor HS were studied. RESULTS: HS was detected in interstitial fibrosis and in advanced glomerulosclerosis, whereas bFGF-binding domains were found only in the fibrosis: The distributional pattern of the N- sulfate-enriched and O- sulfate-scarce portions of native HS was similar to that of bFGF-binding domains. Moreover, a small population of parenchymal cells in advanced tubulointerstitial fibrosis with marked cellular infiltration were especially rich in the bFGF-binding domains. CONCLUSIONS: In fibrotic lesions of the peritubular interstitium, HS shows enrichment of bFGF-binding domains. These regions may play an important role in the fibrogenesis through their interaction with endogenous bFGF.
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Authors | H Morita, T Shinzato, G David, A Mizutani, H Habuchi, Y Fujita, M Ito, J Asai, K Maeda, K Kimata |
Journal | Laboratory investigation; a journal of technical methods and pathology
(Lab Invest)
Vol. 71
Issue 4
Pg. 528-35
(Oct 1994)
ISSN: 0023-6837 [Print] United States |
PMID | 7967508
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fibroblast Growth Factor 2
- Heparitin Sulfate
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Topics |
- Adult
- Female
- Fibroblast Growth Factor 2
(analysis, metabolism, physiology)
- Glomerulosclerosis, Focal Segmental
(metabolism, pathology)
- Heparitin Sulfate
(analysis, chemistry, metabolism)
- Humans
- Immunohistochemistry
- Kidney Glomerulus
(chemistry, metabolism, pathology)
- Male
- Middle Aged
- Nephritis, Interstitial
(metabolism, pathology)
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