The use of an indirect ELISA with protein G-peroxidase conjugate and a blocking ELISA to demonstrate recent bluetongue infection in sheep.

Abstract

The humoral immune response of sheep infected with bluetongue virus serotypes 3, 9 and 16 was monitored by plaque inhibition (PI), blocking ELISA (BELISA) and indirect ELISA over a period of 63 days post-infection. Results indicated that testing of a single plasma or serum sample by both a BELISA and an indirect ELISA using a recombinant streptococcal protein G (PrG) peroxidase conjugate enabled an assessment of the proximity of a recent infection based on the failure of PrG to bind ovine IgM class antibodies. When BELISA and indirect ELISA results were expressed as a ratio, values indicative of recent infection (> or = 5) were observed for an average duration of 16.5 days (range 8 to 23 days) following the initial detection of antibody by BELISA. This approach has potential to improve diagnosis of a wide range of virus infections by providing an indicator for the relationship of serological status with a recent infection. However, where reinfection may occur, as with bluetongue virus, alternative methods may be required for definitive diagnosis.

• Authors: R A Lunt, S D Blacksell, K M Newberry
• Journal: Journal of virological methods (J Virol Methods) Vol. 48 Issue 1 Pg. 53-63 (Jun 1994) ISSN: 0166-0934 [Print] Netherlands
• PMID: 7962260 (Publication Type: Journal Article)

Chemical References

• Antibodies, Viral

Topics

• Animals
• Antibodies, Viral (biosynthesis, blood)
• Bluetongue (blood, diagnosis, immunology)
• Bluetongue virus (classification, immunology)
• Convalescence
• Enzyme-Linked Immunosorbent Assay (methods)
• Male
• Serotyping
• Sheep (immunology)
• Time Factors