Synaptotagmin I is essential for Ca(2+)-independent release of neurotransmitter induced by alpha-latrotoxin.

Abstract

alpha-Latrotoxin causes massive release of norepinephrine from clonal rat pheochromocytoma PC12 cells, in the absence of external Ca2+, by an unknown mechanism. The effect almost disappeared in PC12 variant cells deficient in synaptotagmin I, a synaptic vesicle protein, and was rescued by transfecting the synaptotagmin I gene. These results indicate that synaptotagmin I is essential for the Ca(2+)-independent action of alpha-latrotoxin in PC12 cells.

• Authors: Y Shoji-Kasai, A Yoshida, A Ogura, R Kuwahara, A Grasso, M Takahashi
• Journal: FEBS letters (FEBS Lett) Vol. 353 Issue 3 Pg. 315-8 (Oct 24 1994) ISSN: 0014-5793 [Print] England
• PMID: 7957883 (Publication Type: Journal Article)

Chemical References

• Calcium-Binding Proteins
• Culture Media
• Membrane Glycoproteins
• Nerve Tissue Proteins
• Spider Venoms
• Synaptotagmin I
• Syt1 protein, rat
• Synaptotagmins
• alpha-latrotoxin
• Potassium
• Calcium
• Norepinephrine

Topics

• Animals
• Calcium (physiology)
• Calcium-Binding Proteins
• Culture Media
• Membrane Glycoproteins (genetics, physiology)
• Nerve Tissue Proteins (genetics, physiology)
• Norepinephrine (metabolism)
• PC12 Cells
• Potassium (pharmacology)
• Rats
• Spider Venoms (pharmacology)
• Synaptotagmin I
• Synaptotagmins
• Transfection