Modulation of integrin-laminin receptor function on mammary tumor cells by prostaglandin E2 receptor antagonism.

Abstract

Our previous studies indicate that prostaglandin E2 (PGE2) receptors play a role in tumor metastasis. We asked if PGE2 receptor antagonism would affect murine mammary tumor cell attachment to immobilized laminin, a critical step in metastasis. The PGE2 receptor antagonist, LEO101, at a concentration of 20 micrograms/ml, inhibited tumor cell attachment to laminin and the laminin-peptide PA-22 by 41 and 82%, respectively. Immunoprecipitation studies identified the beta 1 integrin subunit as well as the alpha 3 subunit as major membrane components of these cells, whereas little or no alpha 1, alpha 5 or alpha 6 was detected. Antibody blocking studies confirmed that these cells use beta 1, but not the alpha 6 subunit, to attach to laminin. Immunoprecipitation studies of untreated or LEO101-treated cells indicate that the expression of the alpha 3 integrin, but not other integrins, was decreased by LEO101.

• Authors: S Z Zhang, A M Fulton
• Journal: Cancer letters (Cancer Lett) Vol. 85 Issue 2 Pg. 233-8 (Oct 14 1994) ISSN: 0304-3835 [Print] Ireland
• PMID: 7954342 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Integrins
• Peptides
• Receptors, Laminin
• Receptors, Prostaglandin E
• Polyphloretin Phosphate

Topics

• Amino Acid Sequence
• Animals
• Cell Adhesion (drug effects)
• Cells, Cultured
• In Vitro Techniques
• Integrins (metabolism)
• Mammary Neoplasms, Experimental (pathology)
• Mice
• Molecular Sequence Data
• Peptides (chemistry, metabolism)
• Polyphloretin Phosphate (pharmacology)
• Receptors, Laminin (metabolism)
• Receptors, Prostaglandin E (antagonists & inhibitors)