Characterization of a predominantly pistil-expressed gene encoding a gamma-thionin-like protein of Petunia inflata.

Abstract

We isolated a cDNA clone from a pistil cDNA library of Petunia inflata which encodes a protein, PPT, with sequence similarity to gamma-thionins. Characterization of a genomic clone containing a PPT gene revealed the presence of a single intron. Northern analysis revealed that the PPT gene was predominantly expressed in the pistil during all stages of flower development. Since thionins have been implicated in plant defense against pathogens, PPT may play a role similar to that of other defense-related proteins found in the pistil, defending the pistil against pathogen infection.

• Authors: B Karunanandaa, A Singh, T H Kao
• Journal: Plant molecular biology (Plant Mol Biol) Vol. 26 Issue 1 Pg. 459-64 (Oct 1994) ISSN: 0167-4412 [Print] Netherlands
• PMID: 7948892 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA, Complementary
• PPT protein, Petunia inflata
• Plant Proteins
• RNA, Messenger
• RNA, Plant

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA, Complementary (genetics)
• Gene Expression Regulation, Plant
• Gene Library
• Genes, Plant (genetics)
• Molecular Sequence Data
• Plant Proteins (genetics, physiology)
• Plant Stems (chemistry)
• Plants (genetics)
• RNA, Messenger (analysis)
• RNA, Plant (analysis)
• Sequence Alignment
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid