Abstract |
Using isolated rat lungs, we compared prevention of ischemia-reperfusion injury provided by flushing the lungs with modified Euro-Collins solution (EC), University of Wisconsin solution (UW), low- potassium- dextran solution (LPD), or Wallwork solution (WA). After 4 hours' and 6 hours' cold ischemia, reperfusion injury was assessed on the basis of changes in filtration coefficients (Kfc) and pressure-flow curves, characterized by the slope of the curves (incremental resistance) and the extrapolation of this slope to zero flow (pulmonary pressure intercept [Ppi]). After 4 hours, Kfc and Ppi were higher with EC than with UW, LPD, and WA, and the incremental resistance was higher with EC and UW. After 6 hours, Kfc and incremental resistance Ppi were higher with LPD than with WA. Because ischemia-reperfusion injury is associated with decreased endothelial synthesis of prostacyclin and nitric oxide, we tested whether the addition of prostacyclin or the nitric oxide precursor L-arginine to WA would improve preservation. The Kfc and Ppi were lower with both treatments. In conclusion, ischemia-reperfusion injury was best prevented by using WA. The favorable effect of prostacyclin or L-arginine emphasizes the role played by endothelial dysfunction in ischemia-reperfusion injury.
|
Authors | L Xiong, M Mazmanian, A R Chapelier, J Reignier, M Weiss, P G Dartevelle, P Hervé |
Journal | The Annals of thoracic surgery
(Ann Thorac Surg)
Vol. 58
Issue 3
Pg. 845-50
(Sep 1994)
ISSN: 0003-4975 [Print] Netherlands |
PMID | 7944714
(Publication Type: Comparative Study, Journal Article)
|
Chemical References |
- Albumins
- Cardioplegic Solutions
- Chlorides
- Euro-Collins' solution
- Hypertonic Solutions
- Insulin
- Organ Preservation Solutions
- Potassium Compounds
- Propionates
- University of Wisconsin-lactobionate solution
- Wallwork solution
- potassium cardioplegic solution
- Mannitol
- Allopurinol
- Epoprostenol
- Glutathione
- Adenosine
- Raffinose
|
Topics |
- Adenosine
(administration & dosage)
- Albumins
(administration & dosage)
- Allopurinol
(administration & dosage)
- Animals
- Blood Pressure
- Cardioplegic Solutions
(administration & dosage)
- Chlorides
(administration & dosage)
- Epoprostenol
(administration & dosage)
- Glutathione
(administration & dosage)
- Hypertonic Solutions
(administration & dosage)
- Hypothermia, Induced
- In Vitro Techniques
- Insulin
(administration & dosage)
- Lung
(blood supply, physiopathology)
- Lung Compliance
- Male
- Mannitol
(administration & dosage)
- Organ Preservation
(methods)
- Organ Preservation Solutions
- Organ Size
- Potassium Compounds
(administration & dosage)
- Propionates
(administration & dosage)
- Pulmonary Artery
(physiopathology)
- Raffinose
(administration & dosage)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(physiopathology, prevention & control)
- Time Factors
- Vascular Resistance
|