Abstract |
Using an assay of macrophage migration, where the cells emigrate from an agarose droplet, it was found that the neutral proteases trypsin, chymotrypsin, Pronase and elastase have MIF-like activity. Appropriate enzyme inhibitors counteract this effect. To twelve synovial fluids from patients with inflammatory arthritis, which have MIF-like activity (migration index between 0-3 and 0-7) protease indhibitors ( Trasylol, ovomucoid and soybean inhibitor) were added. Ten of the fluids lost some of their MIF-like activity with at least one inhibitor. Phenylmethylsulphonylfluoride counteracted totally the MIF-like activity of the two fluids tested. It is concluded that MIF-like activity of inflammatory synovial fluids is due, at least partially, to proteases.
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Authors | C Geiger, T L Vischer |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 26
Issue 1
Pg. 176-80
(Oct 1976)
ISSN: 0009-9104 [Print] England |
PMID | 793749
(Publication Type: Journal Article)
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Chemical References |
- Antigens
- Macrophage Migration-Inhibitory Factors
- Phenylmethylsulfonyl Fluoride
- Peptide Hydrolases
- Chymotrypsin
- Pancreatic Elastase
- Trypsin
- Pronase
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Topics |
- Animals
- Antigens
- Chymotrypsin
(immunology)
- Female
- Guinea Pigs
- Macrophage Migration-Inhibitory Factors
(antagonists & inhibitors)
- Macrophages
(immunology)
- Mice
- Pancreatic Elastase
(immunology)
- Peptide Hydrolases
(immunology)
- Phenylmethylsulfonyl Fluoride
(pharmacology)
- Pronase
(immunology)
- Synovial Fluid
(immunology)
- Trypsin
(immunology)
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